Measurement

Part:BBa_J48108:Design

Designed by: Laina Magaya   Group: iGEM06_PrairieView   (2006-10-30)


pUC57-Sulfur-m3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9034
    Illegal XbaI site found at 6342
    Illegal PstI site found at 8447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9034
    Illegal NheI site found at 3160
    Illegal NheI site found at 6430
    Illegal PstI site found at 8447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9034
    Illegal BglII site found at 4785
    Illegal BamHI site found at 3099
    Illegal BamHI site found at 5746
    Illegal XhoI site found at 8498
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9034
    Illegal XbaI site found at 6342
    Illegal PstI site found at 8447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9034
    Illegal XbaI site found at 6342
    Illegal PstI site found at 8447
    Illegal NgoMIV site found at 8582
    Illegal AgeI site found at 2934
    Illegal AgeI site found at 3290
    Illegal AgeI site found at 7062
    Illegal AgeI site found at 7174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal SapI site found at 2916


Design Notes

Cut with XbaI, Pst I, EcoRV, SmaI and BamHI


Source

Micrococcus luteus (4698)

References